Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.
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Even though the CV values were not low enough to indicate a high level of precision the SSR markers produced high, and the RAPD markers moderate, correlations between the genetic distance estimates and hybrid performance and heterosis for the BR intrapopulational crosses.
SAS language guide for personal computers. Data analysis Both dominant teinii RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.
Heterotic group assignment and hybrid performance determined by RFLP marker. As expected, the magnitude of the coefficient of variation CV values decreased as the number of polymorphic loci bands evaluated increased. Our results indicate that, apart temnii the RAPD markers, the other DNA marker systems provided consistent information for diversity studies on tropical maize populations and produced genetic distance estimates which were in good agreement. Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred xflp using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.
In the work of Lanza et al.
For each specific number of loci or bands used the polymorphic markers were submitted to random samplings with replacement bootstrap samples and genetic distances were obtained for each bootstrap sample Tivang et al. Rev Bras Genet 4: Brazilian breeding programs have exploited afop genetic diversity of the BR population and demonstrated that high performance cultivars can be obtained from this population Gerage et al.
The correlation coefficient values between genetic distance and hybrid performance for the four markers assayed were similar to the correlation values between genetic distance and heterosis, not only for the inter or intrapopulation crosses but also for all crosses combined. Nucleic Acids Res The results also suggest that the number of loci evaluated should be increased.
Comparison of the genetic distances generated by different molecular markers in diversity studies have been reported by several authors Hahn et al. Although the discrimination power of RFLPs in teknoi studies has been well documented Smith et al. Barbosa I ; Isaias O.
Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
Theor Appl Genet Morgante and Olivieri stated that in soybean the amount of information given by SSR loci in relation to a comparable number of RFLP loci is given by the estimated number of alleles 4. How to cite this article. The box-plots Figure 3 show what happens when the genetic distance CV values, which are different for dominant and codominant markers, are high. The past limitations associated with pedigree data and morphological, aglp and cytological markers for assessing genetic diversity in cultivated and wild plant species teknji largely been circumvented by the development of DNA markers qflp as restriction fragment length polymorphisms RFLPs; Botstein et al.
Correlation of genetic distance with F 1 grain yield and heterosis. Theor Appl Genet 94, To determine the sampling variance of the genetic distances produced by the different molecular data sets we performed bootstrap analysis using a decreasing number of loci for codominant markers or bands for dominant markers.
The AFLP system presents good levels of precision in its adlp estimates and single crosses prediction. However, these molecular markers have technical differences in terms of cost, speed, amount of DNA needed, technical labor, degrees of polymorphism, precision of genetic distance estimates and the statistical power of tests.
Introduction The past limitations associated with pedigree data and morphological, physiological and cytological markers for assessing genetic diversity in cultivated and wild plant species have aaflp been circumvented by the aflpp of DNA markers such as restriction fragment length polymorphisms RFLPs; Botstein et al. Similar patterns were observed for both genetic distance and heterosis. Except for the RAPD markers, all the markers correlated genetic distance with single cross performance and heterosis which showed that they could be useful in predicting single cross performance and heterosis in intrapopulation crosses for broad-based populations.
The Plant J 3: From the analysis of our data it appears that the maximum CV value appears to be, in most cases, the best guarantee for producing reliable estimates of genetic distance. In this method, co-occurrences are divided by the total number of evaluated loci excluding the negative co-occurrences and thus can be interpreted as the proportion of coincidences in relation to the total number of evaluated loci.
Our results indicate that AFLP seemed to be the best-suited molecular assay for fingerprinting and assessing genetic relationships among tropical maize inbred lines with high accuracy.
The SSR markers were promising in terms of the polymorphism and information content revealed, but may involve some additional initial costs associated with primer development.
Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)
Services on Demand Journal. Theor Applied Genet A new technique for DNA fingerprinting. Thirty-two primers showing reproducible polymorphism were selected and used for scoring the 18 inbred lines. Indeed, we found that the RFLP and AFLP markers produced sufficient numbers of polymorphic bands to produce reliable genetic distance estimates with high correlations between these two marker systems, the similarity between the results being teknui by the fact that they are similar techniques based on restriction site changes.
For the AFLP method 20 primer combinations were used and binary scored 1 or 0 with each band being considered a locus while for the SSR method 68 polymorphic primers were used with the binary data being converted into a genotypic matrix which was used to identify alleles and their respective loci.
Plant material and DNA isolation.